Brain-wide mapping of efferent projections of glutamatergic (Onecut3+ ) neurons in the lateral mouse hypothalamus.

AIM: This study mapped the spatiotemporal positions and connectivity of Onecut3+ neuronal populations in the developing and adult mouse brain. METHODS: We generated fluorescent reporter mice to chart Onecut3+ neurons for brain-wide analysis. Moreover, we crossed Onecut3-iCre and Mapt-mGFP (Tau-mGFP) mice to visualize axonal projections. A dual Cre/Flp-dependent AAV construct in Onecut3-iCre cross-bred with Slc17a6-FLPo mice was used in an intersectional strategy to map the connectivity of glutamatergic lateral hypothalamic neurons in the adult mouse. RESULTS: We first found that Onecut3 marks a hitherto undescribed Slc17a6+ /Vglut2+ neuronal cohort in the lateral hypothalamus, with the majority expressing thyrotropin-releasing hormone. In the adult, Onecut3+ /Vglut2+ neurons of the lateral hypothalamus had both intra- and extrahypothalamic efferents, particularly to the septal complex and habenula, where they targeted other cohorts of Onecut3+ neurons and additionally to the neocortex and hippocampus. This arrangement suggests that intrinsic reinforcement loops could exist for Onecut3+ neurons to coordinate their activity along the brain's midline axis. CONCLUSION: We present both a toolbox to manipulate novel subtypes of hypothalamic neurons and an anatomical arrangement by which extrahypothalamic targets can be simultaneously entrained.

Employment Disruption and Wellbeing Among Young Adults: A Cross-National Study of Perceived Impact of the COVID-19 Lockdown.

Young adulthood (18-30 years old) is a crucial period due to its developmental tasks such as career establishment and financial independence. However, young adults' relative lack of resources makes them vulnerable to employment disruptions (job loss and income loss), which may have both immediate and long-term effects on their financial wellbeing and mental health. The economic impact of COVID-19 restrictions resulted in an increase in unemployment and a decrease in income worldwide, especially for young adults. This study examined to what extent and how job loss and income loss due to the pandemic influenced young adults' perception of their present financial wellbeing, future financial wellbeing, and psychological wellbeing by using cross-sectional survey data collected from six countries (China, Italy, Lithuania, Portugal, Slovenia, and the United States). Results showed that the impact of income loss and job loss on all three types of wellbeing were mediated by young adults' negative perception of the COVID-19 lockdown restriction (i.e., perceived as a misfortune). Cross-country differences existed in the key variables. The association between employment disruptions, young adults' perception of the COVID-19 lockdown restriction, and wellbeing were equivalent across countries except China. Implications for policy and practice are discussed.

Profiles of emerging adults' resilience facing the negative impact of COVID-19 across six countries.

Although emerging adults (i.e., individuals aged 18-29 years old) may be at a lesser risk of COVID-19 severe illness and mortality, studies have found that the negative impact of COVID-19 on mental health and well-being is higher among emerging adults when compared to other age groups. The current study aimed to identify profile(s) based on resilience resources, which could help emerging adults in managing the disruptions to their lives following the pandemic. A cross-national sample of 1,768 emerging adults from China, Italy, Lithuania, Portugal, Slovenia, and the US was utilized to identify profiles based on different resilience dimensions (ego-resiliency, positivity, religiosity, socioeconomic status, family support, peer support). Results of the Latent Profile Analysis suggest the presence of four different profiles: no resources, only peer, only family, and well-equipped. The association of these profiles with demographic variables, adulthood markers, self-perceived COVID-19 impact, present well-being, and future life perception was investigated. Implications for resilience theory as well as for future interventions are discussed. Supplementary Information: The online version contains supplementary material available at 10.1007/s12144-022-03658-y.

How culturally unique are pandemic effects? Evaluating cultural similarities and differences in effects of age, biological sex, and political beliefs on COVID impacts.

Despite being bio-epidemiological phenomena, the causes and effects of pandemics are culturally influenced in ways that go beyond national boundaries. However, they are often studied in isolated pockets, and this fact makes it difficult to parse the unique influence of specific cultural psychologies. To help fill in this gap, the present study applies existing cultural theories via linear mixed modeling to test the influence of unique cultural factors in a multi-national sample (that moves beyond Western nations) on the effects of age, biological sex, and political beliefs on pandemic outcomes that include adverse financial impacts, adverse resource impacts, adverse psychological impacts, and the health impacts of COVID. Our study spanned 19 nations (participant N = 14,133) and involved translations into 9 languages. Linear mixed models revealed similarities across cultures, with both young persons and women reporting worse outcomes from COVID across the multi-national sample. However, these effects were generally qualified by culture-specific variance, and overall more evidence emerged for effects unique to each culture than effects similar across cultures. Follow-up analyses suggested this cultural variability was consistent with models of pre-existing inequalities and socioecological stressors exacerbating the effects of the pandemic. Collectively, this evidence highlights the importance of developing culturally flexible models for understanding the cross-cultural nature of pandemic psychology beyond typical WEIRD approaches.

Saving of Freshmen and Their Parents in Slovenia: Saving Motives and Links to Parental Financial Socialization.

Spending money within the budget, financial planning, and saving represent important positive financial behaviors that contribute to financial satisfaction of emerging adults, which in turn predicts their satisfaction with life. In a mixed method study of Slovenian first-year university students (N = 515) and one of their parents, we qualitatively and quantitatively analyzed multi-informant survey data collected online on their motivation to save money and the factors that influence the students' choice (demographics, parental saving, and parental financial socialization). More than half of the freshmen and their parents saved money, mostly for long-term financial goals, larger investments, financial security in the future, and with self-gratification motives. Saving and the motives for saving were moderately associated within the student-parent dyads: The students whose parents saved tended to save themselves, and the saving motives of parents and their emerging adult children also showed similarities. The role of parents as agents of financial socialization in the students' healthy financial behavior was supported by significant associations between the students' recollection of parental socialization practices (direct financial teaching and financial monitoring) and their money saving. Supplementary Information: The online version contains supplementary material available at 10.1007/s10834-021-09789-x.

Molecular design of hypothalamus development.

A wealth of specialized neuroendocrine command systems intercalated within the hypothalamus control the most fundamental physiological needs in vertebrates1,2. Nevertheless, we lack a developmental blueprint that integrates the molecular determinants of neuronal and glial diversity along temporal and spatial scales of hypothalamus development3. Here we combine single-cell RNA sequencing of 51,199 mouse cells of ectodermal origin, gene regulatory network (GRN) screens in conjunction with genome-wide association study-based disease phenotyping, and genetic lineage reconstruction to show that nine glial and thirty-three neuronal subtypes are generated by mid-gestation under the control of distinct GRNs. Combinatorial molecular codes that arise from neurotransmitters, neuropeptides and transcription factors are minimally required to decode the taxonomical hierarchy of hypothalamic neurons. The differentiation of γ-aminobutyric acid (GABA) and dopamine neurons, but not glutamate neurons, relies on quasi-stable intermediate states, with a pool of GABA progenitors giving rise to dopamine cells4. We found an unexpected abundance of chemotropic proliferation and guidance cues that are commonly implicated in dorsal (cortical) patterning5 in the hypothalamus. In particular, loss of SLIT-ROBO signalling impaired both the production and positioning of periventricular dopamine neurons. Overall, we identify molecular principles that shape the developmental architecture of the hypothalamus and show how neuronal heterogeneity is transformed into a multimodal neural unit to provide virtually infinite adaptive potential throughout life.

Development of the Short Version of the Individuation Test for Emerging Adults (ITEA-S) and Its Measurement Invariance Across Slovene and U.S. Emerging Adults.

In this study we developed a short version of the Individuation Test for Emerging Adults (ITEA-S) and tested its measurement invariance across Slovene and U.S. samples of emerging adults. The item reduction process resulted in retaining 21 out of 36 items. The content analysis of the retained and discarded items revealed that the ITEA-S adequately measures the 5 individuation domains (support seeking, connectedness, intrusiveness, self-reliance, and fear of disappointing the parent). In our samples, the ITEA-S demonstrated evidence for construct validity and its 5 scales showed adequate internal consistency. The scale scores were also meaningfully associated with the relevant demographic variables in both countries, as well as the three scales of the Psychological Separation Inventory (Hoffman, 1984 ; the Slovene sample), and the scales of the Differentiation of Self-Revised questionnaire (Skowron & Schmitt, 2003 ; the U.S. sample). The results of multiple group confirmatory factor analyses supported the hypothesis of full metric and partial scalar invariance for both ITEA-S forms (in relation to mother and father) across the 2 country groups.

Individuation in Slovene emerging adults: its associations with demographics, transitional markers, achieved criteria for adulthood, and life satisfaction.

The study investigated associations of Slovene emerging adults' age, gender, living situation, romantic relationship, and employment status with aspects of individuation in relation to mother and father. Controlling for demographic variables and transitional markers of adulthood, we further explored the contribution of individuation measures to individuals' perceptions of achieved criteria for adulthood and life satisfaction. The participants provided self-reports on the Individuation Test for Emerging Adults, the Satisfaction With Life Scale, and the list of Achieved Criteria for Adulthood. Age and living out of parental home were positively associated with self-reliance in relation to both parents, whereas female gender was related to higher levels of connectedness and seeking parental support. Along with age and involvement in a romantic relationship, connectedness and self-reliance predicted adulthood criteria attainment and life satisfaction. The results support the models of individuation that emphasize growing autonomy and retaining connectedness to parents as pathways towards personal adjustments.

Student personality traits predicting individuation in relation to mothers and fathers.

The role of personality traits in 674 emerging adult students' (aged 18 to 28; 80% female) individuation in relation to parents was investigated cross-sectionally. Self-reports were obtained by the Big Five Inventory and the Individuation Test for Emerging Adults. Personality was predictive of measures of individuation, over and above the students' background characteristics, suggesting that personality can be viewed as an inner resource shaping experiences of individuation. Agreeableness contributed to support seeking, and connectedness with both parents, and Extraversion predicted connectedness with mothers. Conscientiousness was related negatively to both perceptions of parental intrusiveness and fear of disappointing the mother, whereas Neuroticism was predictive of perceptions of maternal intrusiveness, and fear of disappointing the parents. Openness was associated with self-reliance in relationships with both parents, and demonstrated negative links with support seeking and connectedness with mothers. Few moderating effects of age and gender on Extraversion-individuation associations were revealed.

Development and construct validation of the Individuation Test for Emerging Adults (ITEA).

This article presents the construction, exploratory dimensionality analysis (Study 1), and validation (Study 2) of the self-report and 36-item Individuation Test for Emerging Adults (ITEA). It is the first instrument to measure different aspects of individuation (in relation to mother and father) specifically in emerging adulthood. The construct validity of the final 5-factor structure (Support Seeking, Connectedness, Intrusiveness, Self-Reliance, and Fear of Disappointing the Parent) was satisfactory. The results of multigroup analyses demonstrated that the factor structure is stable across male and female emerging adults for ITEA assessments in relation to both mother and father. The ITEA scales also show good internal consistency and concurrent validity against Emotional, Conflictual, and Functional Dependence scales of the Psychological Separation Inventory.

Reliability and validity evidence of scores on the Slovene version of the questionnaire about interpersonal difficulties for adolescents.

This study analyzed the psychometric properties of scores on the Slovene version of the Questionnaire about Interpersonal Difficulties for Adolescents (QIDA) in a sample of 1,334 adolescents (44% boys), ranging in age from 12 to 18 years (M = 15.61). Confirmatory factor analyses replicated the correlated five-factor structure of the QIDA: Assertiveness, Heterosexual Relationships, Public Speaking, Family Relationships, and Close Friendships. Internal consistency and test-retest reliability were reasonable. Correlations of scores on the QIDA with scores of neuroticism, low extraversion, and low openness, as measured by the Inventory of Child/Adolescent Individual Differences, and scores of fear of negative evaluation, and tension and inhibition in social contacts, as measured by the Social Anxiety Scale for Adolescents were found, revealing differential links with QIDA subscale scores. Girls reported more difficulties than boys. Age differences showed a small but significant decrease in QIDA total score over adolescence.

Self-reported adherence behavior in adolescent hypertensive patients: the role of illness representations and personality.

OBJECTIVE: This exploratory study examined the role that illness representations and personality play in the various adherence behaviors of adolescents diagnosed with essential hypertension. METHODS: The participants were 97 hypertensive adolescents. They completed self-report questionnaires pertaining to (1) demographic and medical data, (2) adherence, (3) illness representations, and (4) personality. Medical charts were also assessed. RESULTS: The hierarchical regression analyses indicated that: (1) conscientiousness, agreeableness, and perception of treatment effectiveness account for a significant amount of variance in general adherence; (2) perception of treatment effectiveness is predictive of overall specific adherence; and (3) for adherence to most of the individual specific regimen recommendations, illness representations are more predictive compared to personality dimensions. CONCLUSIONS: The personality domains of conscientiousness, extraversion, agreeableness, and illness representation dimensions (treatment control, concern, and emotional burden) were shown to predict adherence behaviors in adolescent hypertensive patients differentially. Study implications and limitations are discussed.